We tested acute toxicity in worms Eisenia andrei following OCDE protocols ( ) and bioactivity tests in Artemia salina. The alkaloids fraction. Bioensayo de toxicidad en artemia salina. Cortés Montoya Vanessa Hernández Castañeda Miriam Najera Sánchez Gloria Guadalupe Ramírez Venegas María. Bioensayo de La Toxicidad Del Nitrato de Potasio en Reproductores de Artemia Salina en Condiciones de Laboratorio – Download as Word Doc .doc), PDF.

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Taxonomic identification was performed through cellular scale characterization, using transmission electron microscopy Seoane et al.

Química y biología del extracto etanólico del epicarpio de Crescentia cujete L. (totumo)

No significant differences in mortality between control and samples were observed. On the other hand, larvae were considered non-well-developed when no four arms were formed Fig. The most common is the mouse bioassay developed by the Japanese Ministry of Health txicidad Welfare Yasumoto et al.

Artemia franciscana tests were performed following the standard operational procedure Artoxkit Artoxkit M. Nevertheless, it was found that the cytotoxicity of DESs was much higher than artsmia individual components e.

Alexandrium minutum Halim is well known for the production of paralytic shellfish poisoning toxins and its presence can affect copepods, shellfish, and other marine organisms Zhenxing et al.

Due to the high toxicity reported for A. Comparison of methods to obtain biiensayo liquid phase in marine sediment toxicity bioassays with Paracentrotus lividus sea urchin embryos. Organophosphates Search for additional papers on this topic.

Artemia salina

Preliminary survey of toxicity of the green alga Caulerpa taxifolia introduced intro the Mediterranean. Also, the effects of pH and temperature on amylase activity and stability were investigated. Initial test 0; ; ; ; ; ; Definitive test 0; ; ; ; ; C. Roma, Italy, pp. Nevertheless, our results place P. Considering the filtrate of P. After that time, all larvae had moulted to instar II-III stages and the hatched nauplii were separated from remaining cysts using a Pasteur pipette.

Therefore, besides working on the use of the bioassays described in the present work, we also suggest the use of alternative methodologies in order to explore a wider range of tests for the detection of toxic events. The toxic effect of A. These crustaceans are characterized by their adaptability to a wide range of salinity and temperature conditions: The bioassays were performed on II-III stage nauplii larvae of Artemia franciscanaon newly-fertilized sea urchin embryos Paracentrotus lividusand on adults of the amphipod Corophium multisetosum.


Differences were considered as significant when p was lower than 0.

Dead larvae were observed under the microscope in order to determine the presence or absence of algae cells inside the digestive system. Three methanol extracts showed antioxidant activity; three extracts showed insecticidal activity and the presence of terpenic substances and five extracts presented cytotoxic activity, but it was not possible to correlate it with any specific secondary metabolite. Tests of animal exposure to different concentrations of the toxicant were performed for 24, 48 and 72 hours after larvae hatching.

Our results have therefore confirmed the good quality of the model organisms differences in the quality of gonad material were observed in the two different periods where sea urchins were collected, as the LC 50 value for SDS in P. Moreover, Zhenxing et al. Retos ante el tratado de libre comercio. Feeding and survival rates of the copepods Euterpina acutifrons Dana and Acartia grani Sars on the dinoflagellates Alexandrium minutum Balech and Gyrodinium corsicum Paulmier and the Chryptophyta Rhodomonas baltica Karsten.

These findings suggest that the use of different organizational biological level bioassays appears to be a suitable tool for A.

In this work, the cytotoxicity and toxicity toxicodad phosphonium-based deep eutectic solvents DESs with three hydrogen bond donors, namely glycerine, ethylene glycol, and triethylene glycol were investigated.

Effect of Caulerpa taxifolia secondary metabolites on the embryogenesis, larval development and metamorphosis of the sea urchin Paracentrotus lividus.

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Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. In continuation of investigation for environmentally benign protocol for new solvents termed deep eutectic solvents DESsit bioensaho herein reported results concerning the toxicity and cytotoxicity of choline chloride ChCl based DESs with four hydrogen bond donors including glycerine, ethylene glycol, triethylene glycol and urea.


These investigators observed that Prymensium cells were attached to most exterior surfaces, to fish gills and to feeding and respiratory appendages in zooplankton, which contributed to the movement depletion of individuals.

Enter the email address you signed up with and we’ll email you a reset link. From This Paper Figures, tables, and topics from this paper. The bioassay was based on the exposure of the newly-fertilized eggs to different concentrations of cultured and filtered microalgae for 48 hours Table 1. Los extractos activos de D.

The active extracts of D. Ej this research field, phytoplankton toxicity has been tested in marine organisms, including fish and invertebrates; the tests include mortality tests with crustaceans of the genus Artemia Houdan et al. In addition, the minimum concentration with significant mortality was cell mL —1 at 24 and 48 hours of exposure.

We suggest performing the bioassays of the present study with the model organisms, using recent isolated A.

Análisis de cloruros by Javier Roman Nava on Prezi

Phytochemical screening and standardization of poly herbal formulation for dyslipidemia. Moreover, toxicity of extracts from different diatoms has been evaluated in sea urchin embryos Buttino et al. The cytotoxicity effect was A, equatorial dissection of the sea urchin before fertilization; B, oocyte with the fertilization membrane after fertilization; C, sea urchin larva completely developed with the four arms correctly separate; D, poorly developed sea urchin larva resulting after exposure to toxic sample.

Bioactive furanonaphthoquinones from Crescentia cujete. For the taxonomic identification, cells were stained with calcofluor 0.

Therefore, the minimum toxic concentration values established in the present study for the artejia organisms cannot be considered as a real toxic value. Toxic effects of harmful benthic dinoflagellate Ostreopsis ovata on invertebrate and vertebrate marine organisms.